Systemic oxidative stress in victims of Bothrops snakebites
Systemic oxidative stress in victims of Bothrops snakebites
Data
2015
Autores
Strapazzon, Juliana de Ornellas
Parisotto, Eduardo Benedetti
Moratelli, Ana Maria
Garlet, Thais Regina
Bastos, Juliana
Zimermann, Ivan Ricardo
Zanin, Marlene
Fagundez, Rodrigo
Lino, Manuel Rosa de Oliveira
Fröde, Tânia Silvia
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Applied Biomedicine
Resumo
The genus Bothrops is responsible for approximately 90% of snakebites in Brazil. In the present
study biomarkers of oxidative stress (OS) were evaluated in the blood of victims of snakebites
fromBothrops jararaca andBothrops jararacussu. Patientmonitoring started from the emergency
entrance at the hospital up to 30 days, groups divided as follows: time 0 (t0), 24 hours (t24h), 7
days (t7d) and 30 days (t30d). The activities of catalase (CAT), superoxide dismutase (SOD),
glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), and
myeloperoxidase (MPO), as well as the contents of reduced glutathione (GSH), vitamin E, lipid
peroxidation (TBARS), protein carbonyls (PC) were examined in blood. Initial determinations
revealed increased CAT, GR and GPx activities and decreased SOD and GST activities together
with the depletion of GSH contents, while markers of oxidative damage showed increased
TBARS levels and decreased PC concentrations in victims of snakebite compared to controls
(blood donors). Regarding the temporal effect, no statistical differences among the groupswere
detected for the distinct parameters analyzed. The responses obtained in OS biomarkers in
victims of snakebite compared to healthy subjects indicate that Bothrops envenomation
promoted a pronounced and persistent systemic OS in the blood of those subjects.
Description
Palavras-chave
Snakebites, Bothrops, Ophidic accidents, Inflammation, Oxidative stress, Antioxidants
Citação
Ornellas Strapazzon J, Benedetti Parisotto E, Moratelli AM, Garlet TR, Bastos J, Zimermann IR, et al.. Systemic oxidative stress in victims of Bothrops snakebites. J Appl Biomed. 2015;13(2):161-167. doi: 10.1016/j.jab.2014.11.002.