Viability of Human Adenovirus from Hospital Fomites
Viability of Human Adenovirus from Hospital Fomites
Data
2014
Autores
Ganime, Ana Carolina
Carvalho-Costa, Filipe A.
Santos, Marisa
Filho, Rubens Costa
Leite, José Paulo G.
Miagostovich, Marize P.
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Medical Virology
Resumo
The monitoring of environmental microbial
contamination in healthcare facilities may be a
valuable tool to determine pathogens transmis-
sion in those settings; however, such proce-
dure is limited to bacterial indicators. Viruses
are found commonly in those environments
and are rarely used for these procedures. The
aim of this study was to assess distribution
and viability of a human DNA virus on fomites
in an Adult Intensive Care Unit of a private
hospital in Rio de Janeiro, Brazil. Human
adenoviruses (HAdV) were investigated in 141
fomites by scraping the surface area and
screening by quantitative PCR (qPCR) using
TaqMan1 System (Carlsbad, CA). Ten positive
samples were selected for virus isolation in
A549 and/or HEp2c cell lines. A total of 63
samples (44.7%) were positive and presented
viral load ranging from 2.48 101 to 2.1 103
genomic copies per millilitre (gc/ml). The via-
bility was demonstrated by integrated cell
culture/nested-PCR in 5 out of 10 samples.
Nucleotide sequencing confirmed all samples
as HAdV and characterized one of them as
specie B, serotype 3 (HAdV-3). The results
indicate the risk of nosocomial transmission
via contaminated fomites and point out the
use of HAdV as biomarkers of environmental
contamination.
Description
Palavras-chave
adenovirus, viability, fomites, hospital, biomarker
Citação
Ganime AC, Carvalho-Costa FA, Santos M, Costa Filho R, Leite JP, Miagostovich MP. Viability of human adenovirus from hospital fomites. J Med Virol. 2014 Dec;86(12):2065-9. doi: 10.1002/jmv.23907