Viability of Human Adenovirus from Hospital Fomites
Viability of Human Adenovirus from Hospital Fomites
| dc.contributor.author | Ganime, Ana Carolina | |
| dc.contributor.author | Carvalho-Costa, Filipe A. | |
| dc.contributor.author | Santos, Marisa | |
| dc.contributor.author | Filho, Rubens Costa | |
| dc.contributor.author | Leite, José Paulo G. | |
| dc.contributor.author | Miagostovich, Marize P. | |
| dc.date.accessioned | 2024-10-31T18:44:48Z | |
| dc.date.available | 2024-10-31T18:44:48Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | The monitoring of environmental microbial contamination in healthcare facilities may be a valuable tool to determine pathogens transmis- sion in those settings; however, such proce- dure is limited to bacterial indicators. Viruses are found commonly in those environments and are rarely used for these procedures. The aim of this study was to assess distribution and viability of a human DNA virus on fomites in an Adult Intensive Care Unit of a private hospital in Rio de Janeiro, Brazil. Human adenoviruses (HAdV) were investigated in 141 fomites by scraping the surface area and screening by quantitative PCR (qPCR) using TaqMan1 System (Carlsbad, CA). Ten positive samples were selected for virus isolation in A549 and/or HEp2c cell lines. A total of 63 samples (44.7%) were positive and presented viral load ranging from 2.48 101 to 2.1 103 genomic copies per millilitre (gc/ml). The via- bility was demonstrated by integrated cell culture/nested-PCR in 5 out of 10 samples. Nucleotide sequencing confirmed all samples as HAdV and characterized one of them as specie B, serotype 3 (HAdV-3). The results indicate the risk of nosocomial transmission via contaminated fomites and point out the use of HAdV as biomarkers of environmental contamination. | |
| dc.identifier.citation | Ganime AC, Carvalho-Costa FA, Santos M, Costa Filho R, Leite JP, Miagostovich MP. Viability of human adenovirus from hospital fomites. J Med Virol. 2014 Dec;86(12):2065-9. doi: 10.1002/jmv.23907 | |
| dc.identifier.other | DOI: 10.1002/jmv.23907 | |
| dc.identifier.uri | https://dspace.inc.saude.gov.br/handle/123456789/571 | |
| dc.language.iso | en | |
| dc.publisher | Journal of Medical Virology | |
| dc.subject | adenovirus | en |
| dc.subject | viability | en |
| dc.subject | fomites | en |
| dc.subject | hospital | en |
| dc.subject | biomarker | en |
| dc.title | Viability of Human Adenovirus from Hospital Fomites | |
| dc.type | Article |