Group A rotavirus detection on environmental surfaces in a hospital intensive care unit

Ganime, Ana Carolina
Carvalho-Costa, Filipe A.
Mendonça, Marcos Cesar L.
Vieira, Carmen B.
Santos, Marisa
Costa Filho, Rubens
Miagostovich, Marize P.
Leite, José Paulo G.
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American Journal of Infection Control
Background: Environmental surfaces can play a role in the spread of pathogens, such as enteric viruses, within a hospital. This study assessed the level of contamination of group A rotavirus (RV-A) on environmental surfaces samples from an adult intensive care unit in a hospital in Rio de Janeiro, Brazil. Methods: A total of 504 environmental surface samples were obtained from multiple sites in the intensive care unit, including flushing buttons, telephones, and alcohol gel supports. Nested and quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) were used to detect and quantify RV-A levels through partial amplification of VP6 and NSP3 genes, respectively, and the viability of the viruses detected was assessed by MA-104 cell integrated cell culture/RT-PCR. Results: RV-A was detected by nested RT-PCR in 14% of the samples (73 of 504), with viral loads ranging from 3.4 genomic copies/mL to 2.9 x 103 genomic copies/mL. The nucleotide sequence of the amplicons obtained from nested RT-PCR confirmed that the positive samples were RV-A. Moreover, 3 of 10 strains investigated demonstrated viability by integrated cell culture/RT-PCR. Conclusion: The detection of RV-A on environmental surface samples indicates a need for improvements to hospital cleaning procedures to reduce viral contamination, and suggests, as reported previously, that RV-A can be used as a biomarker to assess contamination in hospitals.
Adult ward, Hospital infection, Virus biomarker, Fomites
Ganime AC, Carvalho-Costa FA, Mendonça MC, Vieira CB, Santos M, Costa Filho R, Miagostovich MP, Leite JP. Group A rotavirus detection on environmental surfaces in a hospital intensive care unit. Am J Infect Control. 2012 Aug;40(6):544-7. doi: 10.1016/j.ajic.2011.07.017. Epub 2011 Oct 22. PMID: 22018841.