Group A rotavirus detection on environmental surfaces in a hospital intensive care unit
Group A rotavirus detection on environmental surfaces in a hospital intensive care unit
Data
2012
Autores
Ganime, Ana Carolina
Carvalho-Costa, Filipe A.
Mendonça, Marcos Cesar L.
Vieira, Carmen B.
Santos, Marisa
Costa Filho, Rubens
Miagostovich, Marize P.
Leite, José Paulo G.
Journal Title
Journal ISSN
Volume Title
Publisher
American Journal of Infection Control
Resumo
Background: Environmental surfaces can play a role in the spread of pathogens, such as enteric viruses,
within a hospital. This study assessed the level of contamination of group A rotavirus (RV-A) on environmental surfaces samples from an adult intensive care unit in a hospital in Rio de Janeiro, Brazil.
Methods: A total of 504 environmental surface samples were obtained from multiple sites in the
intensive care unit, including flushing buttons, telephones, and alcohol gel supports. Nested and quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) were used to detect and quantify RV-A
levels through partial amplification of VP6 and NSP3 genes, respectively, and the viability of the viruses
detected was assessed by MA-104 cell integrated cell culture/RT-PCR.
Results: RV-A was detected by nested RT-PCR in 14% of the samples (73 of 504), with viral loads ranging
from 3.4 genomic copies/mL to 2.9 x 103 genomic copies/mL. The nucleotide sequence of the amplicons
obtained from nested RT-PCR confirmed that the positive samples were RV-A. Moreover, 3 of 10 strains
investigated demonstrated viability by integrated cell culture/RT-PCR.
Conclusion: The detection of RV-A on environmental surface samples indicates a need for improvements
to hospital cleaning procedures to reduce viral contamination, and suggests, as reported previously, that
RV-A can be used as a biomarker to assess contamination in hospitals.
Description
Palavras-chave
Adult ward, Hospital infection, Virus biomarker, Fomites
Citação
Ganime AC, Carvalho-Costa FA, Mendonça MC, Vieira CB, Santos M, Costa Filho R, Miagostovich MP, Leite JP. Group A rotavirus detection on environmental surfaces in a hospital intensive care unit. Am J Infect Control. 2012 Aug;40(6):544-7. doi: 10.1016/j.ajic.2011.07.017. Epub 2011 Oct 22. PMID: 22018841.