Mechanisms of kidney repair by human mesenchymal stromal cells after ischemia: A comprehensive view using label-free MSE

Página do item simplificado
dc.contributor.authorCosta, Milene R. da
dc.contributor.authorPizzatti, Luciana
dc.contributor.authorLindoso, Rafael S.
dc.contributor.authorSant’Anna, Julliana Ferreira
dc.contributor.authorDuRocher, Barbara
dc.contributor.authorAbdelhay, Eliana
dc.contributor.authorVieyra, Adalberto
dc.date.accessioned2024-11-01T16:46:28Z
dc.date.available2024-11-01T16:46:28Z
dc.date.issued2014
dc.description.abstractAcute kidney injury (AKI) is one of the more frequent and lethal pathological conditions seen in intensive care units. Currently available treatments are not totally effective but stem cell-based therapies are emerging as promising alternatives, especially the use of mesenchymal stromal cells (MSC), although the signaling pathways involved in their beneficial actions are not fully understood. The objective of this study was to identify signaling networks and key proteins involved in the repair of ischemia by MSC. Using an in vitro model of AKI to investigate paracrine interactions and label-free high definition 2D-NanoESI-MSE, differentially expressed proteins were identified in a human renal proximal tubule cell lineage (HK-2) exposed to hu- man MSC (hMSC) after an ischemic insult. In silico analysis showed that hMSC stimulated antiapoptotic activity, normal ROS handling, energy production, cytoskeleton organization, protein synthesis, and cell proliferation. The proteomic data were validated by parallel experi- ments demonstrating reduced apoptosis in HK-2 cells and recovery of intracellular ATP levels. qRT-PCR for proteins implicated in the above processes revealed that hMSC exerted their effects by stimulating translation, not transcription. Western blotting of proteins associated with ROS and energy metabolism confirmed their higher abundance in HK-2 cells exposed to hMSC.
dc.identifier.citationCosta MR, Pizzatti L, Lindoso RS, Sant'Anna JF, DuRocher B, Abdelhay E, Vieyra A. Mechanisms of kidney repair by human mesenchymal stromal cells after ischemia: a comprehensive view using label-free MS(E). Proteomics. 2014 Jun;14(12):1480-93. doi: 10.1002/pmic.201300084.
dc.identifier.otherDOI: 10.1002/pmic.201300084
dc.identifier.urihttps://dspace.inc.saude.gov.br/handle/123456789/577
dc.language.isoen
dc.publisherProteomics
dc.subjectAcute kidney injuryen
dc.subjectLabel-free quantificationen
dc.subjectMesenchymal stromal cellsen
dc.subjectParacrine communicationen
dc.subjectSignal transduction pathwaysen
dc.subjectTechnologyen
dc.titleMechanisms of kidney repair by human mesenchymal stromal cells after ischemia: A comprehensive view using label-free MSE
dc.typeArticle
Arquivos
Original bundle
Agora exibindo 1 - 1 de 1
thumbnail.default.alt
Nome:
Costa MR et al _Proteomics EN .pdf
Tamanho:
1.7 MB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
License bundle
Agora exibindo 1 - 1 de 1
thumbnail.default.placeholder
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed to upon submission
Descrição:
Baixar
Coleções
Produção Docente