Mechanisms of kidney repair by human mesenchymal stromal cells after ischemia: A comprehensive view using label-free MSE
Mechanisms of kidney repair by human mesenchymal stromal cells after ischemia: A comprehensive view using label-free MSE
Data
2014
Autores
Costa, Milene R. da
Pizzatti, Luciana
Lindoso, Rafael S.
Sant’Anna, Julliana Ferreira
DuRocher, Barbara
Abdelhay, Eliana
Vieyra, Adalberto
Journal Title
Journal ISSN
Volume Title
Publisher
Proteomics
Resumo
Acute kidney injury (AKI) is one of the more frequent and lethal pathological conditions seen in
intensive care units. Currently available treatments are not totally effective but stem cell-based
therapies are emerging as promising alternatives, especially the use of mesenchymal stromal
cells (MSC), although the signaling pathways involved in their beneficial actions are not fully
understood. The objective of this study was to identify signaling networks and key proteins
involved in the repair of ischemia by MSC. Using an in vitro model of AKI to investigate
paracrine interactions and label-free high definition 2D-NanoESI-MSE, differentially expressed
proteins were identified in a human renal proximal tubule cell lineage (HK-2) exposed to hu-
man MSC (hMSC) after an ischemic insult. In silico analysis showed that hMSC stimulated
antiapoptotic activity, normal ROS handling, energy production, cytoskeleton organization,
protein synthesis, and cell proliferation. The proteomic data were validated by parallel experi-
ments demonstrating reduced apoptosis in HK-2 cells and recovery of intracellular ATP levels.
qRT-PCR for proteins implicated in the above processes revealed that hMSC exerted their
effects by stimulating translation, not transcription. Western blotting of proteins associated
with ROS and energy metabolism confirmed their higher abundance in HK-2 cells exposed to
hMSC.
Description
Palavras-chave
Acute kidney injury, Label-free quantification, Mesenchymal stromal cells, Paracrine communication, Signal transduction pathways, Technology
Citação
Costa MR, Pizzatti L, Lindoso RS, Sant'Anna JF, DuRocher B, Abdelhay E, Vieyra A. Mechanisms of kidney repair by human mesenchymal stromal cells after ischemia: a comprehensive view using label-free MS(E). Proteomics. 2014 Jun;14(12):1480-93. doi: 10.1002/pmic.201300084.