Systemic microvascular endothelial dysfunction and disease severity in COVID-19 patients: Evaluation by laser Doppler perfusion monitoring and cytokine/chemokine analysis

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dc.contributor.authorSabioni, Leticia
dc.contributor.authorDe Lorenzo, Andrea
dc.contributor.authorLamas, Cristiane C.
dc.contributor.authorMuccillo, Fabiana B.
dc.contributor.authorCastro-Faria-Neto, Hugo C
dc.contributor.authorEstato, Vanessa
dc.contributor.authorTibirica, Eduardo
dc.date.accessioned2022-09-13T16:49:00Z
dc.date.available2022-09-13T16:49:00Z
dc.date.issued2021
dc.description.abstractBackground Microvascular dysfunction, serum cytokines and chemokines may play important roles in pathophysiology of coronavirus disease 2019 (COVID-19), especially in severe cases. Methods Patients with COVID-19 underwent non-invasive evaluation of systemic endothelium-dependent microvascular reactivity - using laser Doppler perfusion monitoring in the skin of the forearm - coupled to local thermal hyperemia. Maximal microvascular vasodilatation (44 °C thermal plateau phase) was used as endpoint. A multiplex biometric immunoassay was used to assess a panel of 48 serum cytokines and chemokines. Severe COVID-19 (S-COVID) was defined according to WHO criteria, while all other cases of COVID-19 were considered mild to moderate (M-COVID). A group of healthy individuals who tested negative for SARS-CoV-2 served as a control group and was also evaluated with LDPM. Results Thirty-two patients with COVID-19 (25% S-COVID) and 14 controls were included. Basal microvascular flow was similar between M-COVID and controls (P = 0.69) but was higher in S-COVID than in controls (P = 0.005) and M-COVID patients (P = 0.01). The peak microvascular vasodilator response was markedly decreased in both patient groups (M-COVID, P = 0.001; S-COVID, P < 0.0001) compared to the healthy group. The percent increases in microvascular flow were markedly reduced in both patient groups (M-COVID, P < 0.0001; S-COVID, P < 0.0001) compared to controls. Patients with S-COVID had markedly higher concentrations of dissimilar proinflammatory cytokines and chemokines, compared to patients with M-COVID. Conclusions In patients with COVID-19, especially with S-COVID, endothelium-dependent microvascular vasodilator responses are reduced, while serum cytokines and chemokines involved in the regulation of vascular function and inflammation are increased. pten
dc.identifier.citationSabioni L, Lorenzo AD, Lamas CC, Muccillo FB, Castro-FAria-Neto HC, Estato V, Tibiriça E. Systemic microvascular endothelial dysfunction and disease severity in COVID-19 patients: Evaluation by laser Doppler perfusion monitoring and cytokine/chemokine analysis. Microvascular Research. 2021;134:104119.
dc.identifier.doiDOI: 10.1016/j.mvr.2020.104119
dc.identifier.urihttps://dspace.inc.saude.gov.br/handle/123456789/261
dc.language.isoen
dc.publisherMicrovascular Research
dc.subjectLaser Doppler perfusion monitoringen
dc.subjectCOVID-19en
dc.subjectEndothelial dysfunctionen
dc.subjectProinflammatory cytokinesen
dc.subject.meshSeverity of Illness Index
dc.subject.meshPerfusion
dc.subject.meshMiddle Aged
dc.subject.meshMicrocirculation
dc.subject.meshMale
dc.subject.meshLaser-Doppler Flowmetry
dc.subject.meshImmunoassay
dc.subject.meshHumans
dc.subject.meshHemodynamics
dc.subject.meshHealthy Volunteers
dc.subject.meshFemale
dc.subject.meshEndothelium, Vascular / physiopathology
dc.subject.meshCytokines / metabolism
dc.subject.meshCytokines / blood
dc.subject.meshChemokines / metabolism
dc.subject.meshChemokines / blood
dc.subject.meshCOVID-19 / physiopathology
dc.subject.meshAged
dc.subject.meshAdult
dc.titleSystemic microvascular endothelial dysfunction and disease severity in COVID-19 patients: Evaluation by laser Doppler perfusion monitoring and cytokine/chemokine analysisen
dc.typeArticleen
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