Assessment of the IgA Immunoassay Diagnostic Potential of the Mycobacterium tuberculosis MT10.3-MPT64 Fusion Protein in Tuberculous Pleural Fluid

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dc.contributor.authorAraujo, Leonardo Silva
dc.contributor.authorMoraes, Renata Maciel
dc.contributor.authorTrajman, Anete
dc.contributor.authorSaad, Maria Helena Féres
dc.date.accessioned2024-12-09T17:50:19Z
dc.date.available2024-12-09T17:50:19Z
dc.date.issued2010
dc.description.abstractPleural tuberculosis (PL-TB) remains difficult to diagnose. An enzyme-linked immunosorbent assay (ELISA) was developed based on a construction containing the fusion of the Rv3019c (MT10.3) and Rv1980c (MPT64) gene sequences, and its performance was evaluated in an area where TB is endemic. A total of 92 pleural fluid (PF) samples at serial dilutions of 1:50 to 1:800 were included in the ELISA IgA MT10.3-MPT64 evaluation: 70 from TB patients and 22 from patients with other pleurisies. Confirmation of the expression and subsequent purification of the protein was made by SDS-PAGE and Western blot assays, resulting in a 36-kDa protein. ELISA IgA MT10.3-MPT64 showed sensitivities of 61.4%, 58.6%, 62.9%, 67.1%, and 70% at each PF dilution, respectively. The cumulative results of all dilutions increased sensitivity to 81.4% without jeopardizing spec- ificity. Similar results were also obtained at the combined dilutions of 1:50, 1:200, and 1:800 or 1:50 plus 1:800 dilutions (80%). The overall sensitivity of the reference test, i.e., histopathological examination, was 74%. But, via the ELISA IgA MT10.3-MPT64 test, sensitivity was high for specimens with a negative culture (23/27; 85.2%) or nonspecific histopathology (17/18; 94.4%). Our findings demonstrated the promising use of this test as an adjunct in PL-TB diagnoses, particularly in cases with lower bacterial loads and false-negative results in the reference tests, since the new test includes such important features as quick and easy application, high sensitivity and, perhaps most importantly, affordability, which is so crucial for its widespread use in developing countries.
dc.identifier.citationAraujo LS, Maciel RM, Trajman A, Saad MH. Assessment of the IgA immunoassay diagnostic potential of the Mycobacterium tuberculosis MT10.3-MPT64 fusion protein in tuberculous pleural fluid. Clin Vaccine Immunol. 2010 Dec;17(12):1963-9. doi: 10.1128/CVI.00372-10. Epub 2010 Oct 20. Erratum in: Clin Vaccine Immunol. 2011 Feb;18(2):352. Moraes, Renata Maciel [corrected to Maciel, Renata de Moraes].
dc.identifier.otherDOI: 10.1128/CVI.00372-10
dc.identifier.urihttps://dspace.inc.saude.gov.br/handle/123456789/686
dc.language.isoen
dc.publisherCLINICAL AND VACCINE IMMUNOLOGY
dc.subjectAdolescenten
dc.subjectAdulten
dc.subjectAgeden
dc.subjectAged, 80 and overen
dc.subjectAntibodies, Bacterial / analysis*en
dc.subjectAntigens, Bacterial* / geneticsen
dc.subjectAntigens, Bacterial* / immunologyen
dc.subjectEnzyme-Linked Immunosorbent Assay / methodsen
dc.subjectFemaleen
dc.subjectHumansen
dc.subjectImmunoglobulin A / analysis*en
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectMycobacterium tuberculosis / immunology*en
dc.subjectPleural Effusion / immunology*en
dc.subjectRecombinant Fusion Proteins / geneticsen
dc.subjectRecombinant Fusion Proteins / immunologyen
dc.subjectSensitivity and Specificityen
dc.subjectTuberculosis, Pleural / diagnosis*en
dc.subjectYoung Adult.en
dc.titleAssessment of the IgA Immunoassay Diagnostic Potential of the Mycobacterium tuberculosis MT10.3-MPT64 Fusion Protein in Tuberculous Pleural Fluid
dc.typeArticle
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