DNA damage and repair in patients undergoing myocardial perfusion single‐photon emission computed tomography
DNA damage and repair in patients undergoing myocardial perfusion single‐photon emission computed tomography
Data
2024
Autores
Lorenzo, Andrea de
Fernandes, Maria Clara dos Santos
Romeiro, Francisco
Arpini, Anna Paula
Dias, Glauber Monteiro
Journal Title
Journal ISSN
Volume Title
Publisher
Scientifc Reports
Resumo
As patient exposure to ionizing radiation from medical imaging and its risks are continuing issues, this
study aimed to evaluate DNA damage and repair markers after myocardial perfusion single-photon
emission computed tomography (MPS). Thirty-two patients undergoing Tc-99m sestamibi MP
were studied. Peripheral blood was collected before radiotracer injection at rest and 60–90 min after
injection. The comet assay (single-cell gel electrophoresis) was performed with peripheral blood cells
to detect DNA strand breaks. Three descriptors were evaluated: the percentage of DNA in the comet
tail, tail length, and tail moment (the product of DNA tail percentage and tail length). Quantitative
PCR (qPCR) was performed to evaluate the expression of fve genes related to signaling pathways in
response to DNA damage and repair (ATM, ATR, BRCA1, CDKN1A, and XPC). Mann–Whitney’s test
was employed for statistical analysis; p< 0.05 was considered signifcant. Mean Tc-99m sestamibi
dose was 15.1 mCi. After radiotracer injection, comparing post-exposure to pre-exposure samples o
each of the 32 patients, no statistically signifcant diferences of the DNA percentage in the tail, tail
length or tail moment were found. qPCR revealed increased expression of BRCA1 and XPC, without
any signifcant diference regarding the other genes. No signifcant increase in DNA strand breaks was
detected after a single radiotracer injection for MPS. There was activation of only two repair genes,
which may indicate that, in the current patient sample, the efects of ionizing radiation on the DNA
were not large enough to trigger intense repair responses, suggesting the absence of signifcant DNA
damage.
Description
Palavras-chave
Myocardial perfusion imaging, Radiation, Comet assay, DNA.
Citação
De Lorenzo A, Dos Santos Fernandes MC, Romeiro F, Arpini AP, Dias GM. DNA damage and repair in patients undergoing myocardial perfusion single-photon emission computed tomography. Sci Rep. 2024 Jun 7;14(1):13079. doi: 10.1038/s41598-024-63537-3.